解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:The cattle ticks, Boophilus spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant B. microplus Bm86 protective antigen has been shown to protect cattle against tick i...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-29
更新日期:2009-03-31 00:00:00
abstract:BACKGROUND:In spite of a great number of monomeric fluorescent proteins developed in the recent years, the reported fluorescent protein-based FRET pairs are still characterized by a number of disadvantageous features, complicating their use as reporters in cell biology and for high-throughput cell-based screenings. RE...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-9-24
更新日期:2009-03-25 00:00:00
abstract:BACKGROUND:The ability of cytosine deaminase (CD) to convert the antifungal agent 5-fluorocytosine (5-FC) into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU) raised considerable interest in this enzyme to model gene or antibody - directed enzyme-prodrug therapy (GDEPT/ADEPT) a...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-68
更新日期:2008-09-10 00:00:00
abstract:BACKGROUND:Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that episomal retroviral DNA forms are dead-end products of reverse tran...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-60
更新日期:2008-08-09 00:00:00
abstract:BACKGROUND:In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS:Transgeni...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-8-25
更新日期:2008-03-05 00:00:00
abstract:BACKGROUND:Shark heavy chain antibody, also called new antigen receptor (NAR), consists of one single Variable domain (VH), containing only two complementarity-determining regions (CDRs). The antigen binding affinity and specificity are mainly determined by these two CDRs. The good solubility, excellent thermal stabili...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-78
更新日期:2007-11-19 00:00:00
abstract:BACKGROUND:We have investigated the possibility and feasibility of producing the HPV-11 L1 major capsid protein in transgenic Arabidopsis thaliana ecotype Columbia and Nicotiana tabacum cv. Xanthi as potential sources for an inexpensive subunit vaccine. RESULTS:Transformation of plants was only achieved with the HPV-1...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-56
更新日期:2007-09-12 00:00:00
abstract:BACKGROUND:Wax esters are important ingredients in cosmetics, pharmaceuticals, lubricants and other chemical industries due to their excellent wetting property. Since the naturally occurring wax esters are expensive and scarce, these esters can be produced by enzymatic alcoholysis of vegetable oils. In an enzymatic rea...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-53
更新日期:2007-08-30 00:00:00
abstract:BACKGROUND:Plants are increasingly being examined as alternative recombinant protein expression systems. Recombinant protein expression levels in plants from Tobacco mosaic virus (TMV)-based vectors are much higher than those possible from plant promoters. However the common TMV expression vectors are costly, and at ti...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-52
更新日期:2007-08-27 00:00:00
abstract:BACKGROUND:Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, eith...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-37
更新日期:2007-06-29 00:00:00
abstract:BACKGROUND:The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expre...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-29
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND:Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV va...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-12
更新日期:2007-02-26 00:00:00
abstract:BACKGROUND:Dengue is a public health problem of global significance for which there is neither an effective antiviral therapy nor a preventive vaccine. It is a mosquito-borne viral disease, caused by dengue (DEN) viruses, which are members of the Flaviviridae family. There are four closely related serotypes, DEN-1, DEN...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-7-10
更新日期:2007-02-15 00:00:00
abstract:BACKGROUND:Taxol is an effective antineoplastic agent, originally extracted from the bark of Taxus brevifolia with a low yield. Many attempts have been made to produce Taxol by chemical synthesis, semi-synthesis and plant tissue cultures. However, to date, the availability of this compound is not sufficient to satisfy ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-45
更新日期:2006-12-06 00:00:00
abstract:BACKGROUND:A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throu...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-20
更新日期:2006-03-20 00:00:00
abstract:BACKGROUND:The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population. Using thin films of extracellular matrix proteins, we have quantified, on a cell-by-cell basis, phenotypic parameters of...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-14
更新日期:2006-03-06 00:00:00
abstract:BACKGROUND:The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are criti...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-6-8
更新日期:2006-02-10 00:00:00
abstract:BACKGROUND:Whole genome amplification (WGA) promises to eliminate practical molecular genetic analysis limitations associated with genomic DNA (gDNA) quantity. We evaluated the performance of multiple displacement amplification (MDA) WGA using gDNA extracted from lymphoblastoid cell lines (N = 27) with a range of start...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-24
更新日期:2005-09-16 00:00:00
abstract:BACKGROUND:Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS:A method for DNA splicing ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-23
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:DsRed the red fluorescent protein (RFP) isolated from Discosoma sp. coral holds much promise as a genetically and spectrally distinct alternative to green fluorescent protein (GFP) for application in mice. Widespread use of DsRed has been hampered by several issues resulting in the inability to establish and...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-20
更新日期:2005-07-04 00:00:00
abstract:BACKGROUND:Microarray technology has been used extensively over the past 10 years for assessing gene expression, and has facilitated precise genetic profiling of everything from tumors to small molecule drugs. By contrast, arraying cell membranes in a manner which preserves their ability to mediate biochemical processe...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-18
更新日期:2005-06-16 00:00:00
abstract:BACKGROUND:The use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-13
更新日期:2005-05-11 00:00:00
abstract:BACKGROUND:Size exclusion chromatography is the method of choice for separating free from liposome-encapsulated molecules. However, if the column is not presaturated with lipids this type of chromatography causes a significant loss of lipid material. To date, the mechanism of lipid retention is poorly understood. It ha...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-11
更新日期:2005-05-10 00:00:00
abstract:BACKGROUND:The proteomics literature has seen a proliferation of publications that seek to apply the rapidly improving technology of 2D gels to study various biological systems. However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these expe...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-5-7
更新日期:2005-02-11 00:00:00
abstract:BACKGROUND:Advances in optical imaging modalities and the continued evolution of genetically-encoded fluorescent proteins are coming together to facilitate the study of cell behavior at high resolution in living organisms. As a result, imaging using autofluorescent protein reporters is gaining popularity in mouse trans...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-4-33
更新日期:2004-12-24 00:00:00
abstract:BACKGROUND:In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44), kinases (EGFR-cytoplasmic domain, ...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-4-32
更新日期:2004-12-14 00:00:00
abstract:BACKGROUND:The identification of known mutations in a cell population is important for clinical applications and basic cancer research. In this work an immobilized form of the polymerase chain reaction, referred to as polony technology, was used to detect mutations as well as gene deletions, resulting in loss of hetero...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-3-11
更新日期:2003-07-23 00:00:00
abstract:BACKGROUND:Transformed cells of Escherichia coli DH5-alpha with pGFPuv, induced by IPTG (isopropyl-beta-d-thiogalactopyranoside), express the green fluorescent protein (gfpuv) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-p...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-2-7
更新日期:2002-04-24 00:00:00
abstract:BACKGROUND:Hydrophobic polystyrene is the most common material for solid phase immunoassay. Proteins are immobilized on polystyrene by passive adsorption, which often causes considerable denaturation. Biological macromolecules were found to better retain their functional activity when immobilized on hydrophilic materia...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-4
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate w...
journal_title:BMC biotechnology
pub_type: 杂志文章
doi:10.1186/1472-6750-1-11
更新日期:2001-01-01 00:00:00